Kligler iron agar

Specifications

Pectic digest of animal tissue (g/L) 15.00
Beef extract (g/L) 3.00
Yeast extract (g/L) 3.00
Proteose peptone (g/L) 5.00
Lactose (g/L) 10.00
Dextrose (g/L) 1.00
Iron sulphate (g/L) 0.20
Sodium chloride (g/L) 5.00
Sodium thiosulphate (g/L) 0.30
Phenol red (g/L) 0.024

Instruction for use:
Suspnd 57.5 grams in 1000ml distilled
water. Boil to dissolve the medium completely. Mix well &
distribute into tubes. Sterilize by autoclaving at 15 lbs
pressure (121°C) for 15 minutes. Cool the tubes to set as
slopes with 1 inch butts. Best reactions are obtained on
freshly prepared medium. do not use screw-capped tubes or
bottles.