Antibiotic Assay Medium No. 4 (Yeast Beef Agar)

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Specifications

Peptic digest of animal tissue
(peptone) 6.000 G/L
Beef extract 1.500 G/L
Yeast extract 3.000 G/L
Dextrose 1.000 G/L
Agar 15.000 G/L
pH range 6.40 - 6.80
Reaction reaction of 2.65% w/v aqueous
solution at 25°C pH: 6.6 +/- 0.2
Operation :
Suspend 26.5 grams of powder media in 1000 ml of distilled water. Shake well and heat to dissolve
the medium completely. Sterilize by autoclaving at 15 Ibs pressure ( 121°C) for 15 minutes.