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COAs
ALKALINE SALINE PEPTONE WATER
Code: DM2390500
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Specifications
Ingredients g/LPeptone 10.00Sodium chloride 10.00Final pH (at 25°C) : 8.4 + 0.2DirectionSuspend 20.0 grams in 1000 ml distilled water. Heat if necessary, to dissolve the mediumcompletely. Dispense in tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.Principle Peptone provides nitrogen and carbon source, long chain amino acids, vitamins and other essential nutrients. Sodium chloride maintains osmotic equilibriumQC Tests (I) Dehydrated MediumColour : Cream to light yellowAppearance : Homogeneous Free Flowing powder(II) Rehydrated mediumpH (post autoclaving/heating) : 8.4 ± 0.2Colour (post autoclaving/heating) : Pale Yellow to light yellowClarity (post autoclaving/heating) : Clear(III) Q.C. Test MicrobiologicalCultural characteristics observed after 18 –24 hrs at 35-37°C.MICROORGANISM (ATCC ) GROWTHVibrio parahaemolyticus (17802 ) LuxuriantVibrio cholerae (15748 ) LuxuriantUse For enrichment of Vibrio species.Storage Dehydrated medium-below30°C Prepared medium- Between 2 to 8°C.
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