DICHLORAN GLYCEROL MEDIUM BASE - DG18

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Specifications

Ingredients : gms/lit.
Peptic digest of animal tissue 5.00
Dextrose 10.00
Monopotassium phosphate 1.00
Magnesium sulphate 0.50
Chloramphenicol 0.10
Dichloran 0.002
Agar 15.00
Final pH (at 25°C) : 5.6 + 0.2

instruction for use :
Suspend 15.8 grams in 500 ml distilled water. Heat to boiling to dissolve the medium completely. Add 110
grams of glycerol (Analytical Reagent Grade). Sterilize by autoclaving at 15 lbs pressure (121°C) for 15
minutes. Mix well and pour into sterile Petri plates.

Principle :
Dichloran Glycerol Medium is recommended for isolation and enumeration of xerophilic moulds from dried
and semidried foods. Peptic digest of animal tissue provides nitrogen, vitamins and minerals. Dextrose is a
carbohydrate source. Phosphate buffers the medium. Magnesium sulfate provides divalent cations and
sulfate. Dichloran is an antifungal agent, added to the medium to reduce colony diameters of spreading
fungi. The glycerol at 18% (w/w) lowers the water activity Chloramphenicol inhibits gram – negative and
gram – positive bacteria.