Baird parker agar

Specifications

Composition :
Casein enzymic hydrolysate 10.000 G/L
Beef extract 5.000 G/L
Yeast extract 1.000 G/L
Glycine 12.000 G/L
Sodium pyruvate 10.000 G/L
Lithium chloride 5.000 G/L
Agar 20.000 G/L
Final pH (at 25°C) 7.0 +/- 0.2
Operation :
Suspend 63 grams of powder media in 950 ml distilled water. Shake well & heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C and aseptically add 50 ml concentrated Egg Yolk Emulsion and 3 ml sterile 3.5% Potassium Tellurite solution or 50 ml Egg Yolk Tellurite Emulsion. If desired add rehydrated contents of 1 vial of BP Sulpha Supplement . Alternatively 1 vial of Fibrinogen Plasma Trypsin Inhibitor Supplement may be used per 90 ml medium in place of Egg yolk Tellurite Emulsion for identification of coagulase, positive Stapylococci. Mix well and pour into sterile Petri plates. Warning: Lithium Chloride is harmful. Avoid all bodily contact