Ingredients : Tryptone 17.00Proteose peptone 3.00Beef extract 5.00Oxgall 10.00Sodium chloride 5.00Esculin 1.00Ferric ammonium citrate 0.50Sodium azide 0.15Agar 15.00Final pH (at 25°C) : 7.1 + 0.2Principle :This highly nutritious medium because of presence of tryptone, proteose peptone and beef extract.Sodium azide inhibits growth of gram-negative organisms and permits the cultivation of faecalStreptococci. Esculin hydrolysis and bile tolerance permit isolation and identification of group DStreptococci in 24 hours. Agar is the solidifying agent.Directions :Suspend 56.65 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely.Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pourinto sterile Petri plates.