Ingredients : gms/lit.Peptic digest of animal tissue 5.00Dextrose 10.00Monopotassium phosphate 1.00Magnesium sulphate 0.50Chloramphenicol 0.10Dichloran 0.002Agar 15.00Final pH (at 25°C) : 5.6 + 0.2instruction for use : Suspend 15.8 grams in 500 ml distilled water. Heat to boiling to dissolve the medium completely. Add 110grams of glycerol (Analytical Reagent Grade). Sterilize by autoclaving at 15 lbs pressure (121°C) for 15minutes. Mix well and pour into sterile Petri plates.Principle :Dichloran Glycerol Medium is recommended for isolation and enumeration of xerophilic moulds from driedand semidried foods. Peptic digest of animal tissue provides nitrogen, vitamins and minerals. Dextrose is acarbohydrate source. Phosphate buffers the medium. Magnesium sulfate provides divalent cations andsulfate. Dichloran is an antifungal agent, added to the medium to reduce colony diameters of spreadingfungi. The glycerol at 18% (w/w) lowers the water activity Chloramphenicol inhibits gram – negative andgram – positive bacteria.